N the parasitic bioactive proteins including their unique biochemical capabilities and action mechanisms could possibly be useful to establish handle strategies, where the parasitic diseases are prevalent.AddendumDuring the revision course of action, we recognized that genes displaying higher sequence identity with CsGSTo1 andKim et al. Parasites Vectors (2016) 9:Web page 15 ofassayed each 1 h. Growth curves are representative of these from 3 independent experiments. Crucial: , control cells; , CsGSTo1 overexpressing cells; , CsGSTo2 overexpressing cells. (TIF 1468 kb) Abbreviations 2-DE, two-dimensional electrophoresis; 4-NBC, 4-nitrobenzyl chloride; 4-NPA, 4-nitrophenyl acetate; CDNB, 1-chloro-2,4-dinitrobenzene; CHP, cumene hydroperoxide; CsGSTo1, Clonorchis sinensis omega-class GST1; CsGSTo2, Clonorchis sinensis omega-class GST2; CsTub, Cs tubulin; DCNB, 1,2-dichloro4-nitrobenzene; DHA, dehydroascorbate; DHAR, dehydroascorbate reductase; ECL, enhanced chemiluminescence; GSH, decreased glutathione; GST, glutathione transferase; HEDS, hydroxylethyl disulfide; Juglone, 5-hydroxy-1,4naphthoquinone; PBS, phosphate buffered saline; qRT-PCR, quantitative real-time reverse transcription-PCR; SHG, S-hexylglutathione Acknowledgement This work was supported by the Korea National Investigation Foundation (2015R1D1A1A02061866). Authors’ contributions Experimental conception and design and style: YK, JGK, YAB; sample collections: JGK, CSA, NYK, HJY, WMS; cloning and expression: NYK, YAB, JGK, CSA; biochemistry: JGK, CSA, NYK, SHK, IK; immunohistochemistry: SHK, JGK, YAB, NYK; information analysis: YK, JGK, IK, YAB; paper writing: YK, JGK. All authors study and approved the final version with the manuscript. Competing interests The authors declare that they have no competing interests. Ethics approval and consent to participate All protocols like parasite infection, recovery in the worms beneath intraperitoneal injection of 500 l of a 7:three mixture of alfaxlone (ten mg/ml) and xylazine (23.Benzo[d]thiazole-4-carboxylic acid Chemscene 32 mg/ml) and euthanasia with the moribund rats, and production of certain antibodies BALB/c mice, had been authorized by the Institutional Review Board and performed within the Laboratory Animal Investigation Center of Sungkyunkwan University (protocol 2015).5-Bromo-1,2,3,4-tetrahydronaphthalene custom synthesis Author information Department of Molecular Parasitology, Sungkyunkwan University College of Medicine, 2066 Seobu-ro, Jangan-gu, Suwon 16419, Korea. 2Department of Microbiology, Graduate School of Medicine, Gachon University, Incheon, Korea. 3Department of Molecular Biology and Biochemistry, School of Medicine, Kyung Hee University, Seoul, Korea. 4Department of Parasitology, Ewha Womans University, College of Medicine, Seoul, Korea. 5Department of Parasitology and Tropical Medicine, Institute of Overall health Sciences, Gyeongsang National University College of Medicine, Jinju, Korea.PMID:24580853 7.eight.9.ten.11.12. 13.14. 15.16.17.18.19.20.21. Received: six April 2016 Accepted: two JuneReferences 1. Madsen H, Dung BT, The DT, Viet NK, Dalsgaard A, Van PT. The role of rice fields, fish ponds and water canals for transmission of fish-borne zoonotic trematodes in aquaculture ponds in Nam Dinh Province, Vietnam. Parasit Vectors. 2015;eight:625. 2. Keiser J, Utzinger J. Food-borne trematodiases. Clin Microbiol Rev. 2009;22: 4663. three. Marcos LA, Terashima A, Gotuzzo E. Update on hepatobiliary flukes: fascioliasis, opisthorchiasis and clonorchiasis. Curr Opin Infect Dis. 2008;21: 5230. four. Jang KT, Hong SM, Lee KT, Lee JG, Choi SH, Heo JS, et al. Intraductal papillary neoplasm from the bile duct associated with Clonorchis.